Types of agar media pdf
our Handbook of Microbiological Culture Media, revised. and extended, in a new and more attractive format. The range of products has been increased, reafrming the. Scharlau Microbiology brand as an attractive alternative to other manufacturers of culture media for Microbiology.Jul 24, 2010 Any agar media can be made selective by addition of certain inhibitory agents that dont affect the pathogen of interest. Various approaches to make a medium selective include addition of antibiotics, dyes, chemicals, alteration of pH or a combination of these. types of agar media pdf
Agar plates come with many different types of media or nutrients, depending on the microorganism you want to culture. Blood Agar Plates Blood agar plates (BAP) are made by adding five to ten percent sheep or horse blood to the nutrient medium.
Agar is typically used in a final concentration of 12 for solidifying culture media. Smaller quantities ( ) are used in media for motility studies (0. 5 ENRICHMENT, SELECTIVE AND DIFFERENTIAL CULTURE MEDIA Cultivation is the process of propagating microorganisms to grow by providing the proper environmental conditions types of agar media pdf A variety of selective and differential media are used in medical, diagnostic and water pollution laboratories, and in food and dairy laboratories. Three of the more common selective and differential media are described below and will be used in the laboratory exercise. MANNITOL SALT AGAR (MSA) Mannitol salt agar is a selective medium used for the isolation of pathogenic staphylococci.
CULTURE MEDIA FOR FUNGI Introduction A wide range of media are used for growing fungi. Most mycologists develop preferences for certain types of media based on experience and peculiarities of the type of fungi that are routinely grown. Media will affect colony morphology and color, whether Isolation MediaWater Agar, Antibiotic Agar and types of agar media pdf medium makes them enriched media. Blood agar, chocolate agar, Loefflers serum slope etc are few of the enriched media. Blood agar is prepared by adding 510 (by volume) This type of media uses the biochemical characteristics of a microorganism growing in the presence of specific nutrients or indicators (such as neutral red, phenol red Jan 03, 2011 The general purpose media is a media that has a multiple effect, i. e. it can be used as a selective, deferential or a resuscitation media. Isolation Culture Media An isolation culture medium is a simple agar containing solid medium that allows the growth of Microbiological Media Preparation Bacteria and fungi are grown on or in microbiological media of various types. The medium soy agar. These 2 mediaone a liquid and the other a solidare the exact same formula save for the addition of agar agar (reallyagar agar), an extract from the cell walls of You will now add agar to the remaining 95 ml of TSB to produce Tryptic Soy Agar. Like most solid media TSA has an agar concentration of 1. 5 (wv), which is 1. 4 g per 95 ml. 1. Transfer the agar to the TSB solution. 2. Cover the mouth of the flask with foil, and label it on the autoclave tape as TSA with your name and date. 3.Rating: 4.60 / Views: 402